![]() ![]() If necessary, symbols may be explained in a key within the figure image. Splenocytes from DO11.10 mice were incubated with 0, 1, 10, 100, or 1000 ng of MIP-1a per mL (triangles) or MIP-1b (squares) in plates containing 0 (open symbols) or 500 (filled symbols) μg of OVA per ml. If necessary, words may be used to describe the symbols. Symbols represent IVIG treatment groups (n = 4 rats/group): saline ( Symbols should be explained in the text legend if possible, including the symbols themselves if possible. (C) Plasma levels from 10 healthy control (Ctrl) donors.Īny abbreviations used in the figure image but not explained in the article's main text should be defined in the legend. In the example below, “control” should be abbreviated “Ctrl,” not “C,” to avoid confusion with panel C of the figure. Avoid any text labels that might be confused with panel and subpanel labels. ![]() (D) Representative genes that are highly expressed in CMPs.įigure images may contain symbol and text labels that are necessary to convey relevant detail. (C) Representative genes that are highly expressed in CLPs. (B) Representative genes that were upregulated in MPPs. (A) Representative genes that are predominantly expressed in HSCs and downregulated in MPPs, CLPs, and CMPs. The vertical axis represents the normalized gene expression values. Clusters of genes categorized by the expression patterns in purified stem and progenitor cells. A nonboldface description of the figure usually follows, run in after the title, describing each panel, subpanel, inset, or other part of the figure.įigure 4. Do not refer to figure panels, other figure parts, or any other part of an article in a figure title. The figure legend must contain a boldface (a) name ("Figure" + arabic figure number) and (b) substantive title. However, if the tabular material is graphically related to parts of a figure, it can appear within the figure. Tabular material should almost always appear as a table that is not part of a figure. The maximum width is 17.7 cm (2-column width). In order to prevent radical changes in figure content, authors should prepare the figures 8.4 cm wide (1-column width) or, if necessary, 12.7 cm wide (1½ column width). If an article is accepted for publication, the figures may be altered by Blood's publication management vendor to conform to Blood style, which includes, but is not limited to, standard colors and fonts. Images will be sized to fit the smallest possible space while retaining all relevant detail. Protect from light.Images should be laid out as compactly as is consistent with conveying the relevant data. Fulfills highest requirements on antibody validation: structure and function characterized.Strong avidity effect from bivalent form of Spot-VHH.Superior accessibility and labelling of epitopes in crowded cellular/organelle environments.Capture and detection tag without compromises in applications.Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Immunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. STED images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.Īnti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins Description Images were deconvolved with Huygens Professional (SVI). Pixel size: 21 x 21 nm z-Step size of z-Stacks: 0.16 μm. Gated STED images were acquired with a Leica TCS SP8 STED 3X microscope with pulsed White Light Laser excitation at 590 nm and pulsed depletion with a 775 nm laser. STED: IF of Spot-tagged Actin-Chromobody with Spot-Label Atto594 bivalent (1:1,000). ![]() STED images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. ![]()
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